COMPARISON POTENCY ASSAY METHODS AS PLAQUE FORMING UNIT (PFU) AND CELL CULTURE INFECTIOUS DOSE 50% (CCID50) OF THE CANDIDATE NATIONAL REFERENCE STANDARD MEASLES VACCINE

Tóm tắt

A comparative study of two methods (PFU and CCID50) for potency
determination for the national standard candidate measles vaccine was organized at
the National Institute for Control of Vaccines and Biologicals (NICVB). Two
methods are performed on the groups of candidate National Reference Standard
measles vaccine derived from AIK-C strain stored at different temperatures: -700C,
40C/7 days, 370C/7 days. The results show that although there is a difference, strong
correlation exists between the two methods CCID50 and PFU with R2=0,886, r =
0,94, coefficient PFU/CCID50 = 1.28 equivalent to the ratio PFU: CCID50 = 1:0.78.