SPECIFIC INDENTIFICATION OF INFLUENZAVIRRUS (A/H5N1) STRAIN IN INFLUENZA A/H5N1 VACCINE BY RT-PCR METHOD

Tóm tắt

RT-PCR (Reverse transcription - Polymerase Chain Reaction) is applied to
determine the presence of influenza virus A/ H5N1 in influenza A/H5N1vaccine, and
to develop an identity process for specific virus strain A/H5N1 in influenza vaccine
A/H5N1. Selected samples included: Ivacflu-A/H5N1 vaccine (Institute of Vaccines
and Biologicals), Vaxigrip vaccine (Sanofi Pasteur), Influenza virus strain
A/Vietnam/1194/2004(A/H5N1) (NIBSC) was used as positive control; vaccine
Varivax (MSD) and DNA/RNA free water was used as negative controls. The results
of study showed that virus strain A/H5N1 was identified as production of RT-PCR
that were positive with amplified primer pairs of 2 specific gene sequences of HA
whose length 428 and 249 bp.