ANOPHELES SPECIES COMPOSITION AND COMPARISON OF WHO BOTTLE AND TUBE TEST BIOASSAYS FOR EVALUATION SUSCEPTIBILITY OF Anopheles minimus AND Anopheles sinensis MOSQUITOES TO PERMETHRIN IN QUYET CHIEN COMMUNE OF HOA BINH PROVINCE
Từ khóa:
Keywords: Susceptibility, An. minimus, An. sinensis, WHO tube test bioassay, WHO bottle bioassay, permethrin.Tóm tắt
The susceptibility study was conducted in August 2023 at Quyet Chien commune, Tan Lac district, Hoa Binh province. Impregnated paper with permethrin 0,75% and stock solutions of permethrin (21.5 µg per bottle) were used following WHO protocol. The results revealed that a total of 713 individuals of Anopheles mosquitoes belonging to 9 species were collected from the cattle sheds. The main vector An. minimus was sampled with high density 1.99 individuals/person/hour, followed by An. sinensis with the density of 1.78 individuals/person/hour. For both WHO tube test and WHO bottle bioassay methods, An. minimus was susceptible to permethrin with a mortality rate after 24h maintenance of 100%. An. sinensis displayed resistance to permethrin using both WHO tube test and WHO bottle bioassay with the motality was 66% ± 2.31% (Mean ± SD) and 65% ± 3.83% (Mean ± SD) respectively. WHO bottle bioassay should be widely applied to assess the susceptibility of mosquitoes to insecticide in Viet nam.
Keywords: Susceptibility, An. minimus, An. sinensis, WHO tube test bioassay, WHO bottle bioassay, permethrin.
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