VALUE OF THE DIRECT, KATO-KATZ, AND ETHER-FORMALIN TECHNIQUES IN DIAGNOSING INTESTINAL HELMINTHS
Tóm tắt
The study provides more comparative evidence on sensitivity, specificity, convenience, patient acceptance, and cost of stool tests: direct, Kato-Katz, and Ether-Formalin in diagnosing intestinal helminths. We conducted a laboratory-based analytical study design and coordinated qualitative research. Based on the analysis of 190 stool samples, we obtained the following results: The specificity of the Kato-Katz, the direct, and the Ether-Formalin techniques all reached 100%. There are apparent differences in the sensitivity of the above methods. The Kato–Katz technique has the highest sensitivity of 90.00%, while the Ether -Formalin and the direct endoscopy techniques have 80.00% and 70.00%, respectively. Interviews with facilities participating in the study showed that they all implemented the direct stool technique. Still, the Kato-Katz and Ether-Formalin techniques were less commonly deployed for several reasons: the number of patients per day is small, so it takes a long time and complicated processes. 2/5 technicians feel unprepared to conduct the above tests, and all the patients are hesitant when assigned to make a stool test. However, the cost of the tests is considered suitable for most people as laboratory leaders, testing technicians, and patients.
Keywords: intestinal helminths, sensitivity, specificity, direct stool, the Kato-Katz, the Ether-Formalin.
Tài liệu tham khảo
Garcia, L.S. 2007. Diagnostic Medical Parasitology (page 266-270). LSG and associates ASM press, Washington, DC
Những điều cần biết về bệnh giun sán và cách phòng chống. Chương trình mục tiêu quốc gia. Cổng thông tin bộ y tế.
M.R. Tarafder, H. Carabin, L. Joseph, E. Balolong Jr, R. Olveda and .T. McGarvey. 2010. Estimating the sensitivity and specificity of Kato-Katz stool examination technique for detection of hookworms, Ascaris lumbricoides and Trichuris trichiura infections in humans in the absence of a ‘gold standard’. International Journal for Parasitology Volume 40, Issue 4 Pages 399-404. DOI: https://doi.org/10.1016/j.ijpara.2009.09.003 https://doi.org/10.1016/j.ijpara.2009.09.003">
Mulat Yimer, Tadesse Hailu, Wondemagegn Mulu and Bayeh Abera. 2015. Evaluation performance of diagnostic methods of intestinal parasitosis in school age children in Ethiopia. BMC Research Notes. DOI 10.1186/s13104-015-1822-4.
Solomon Taye. 2014. Comparison of Kato-Katz and Formol-Ether Concentration Methods for the Diagnosis of Intestinal Helminthic Infections among School Children of Wonji Shoa Town, Eastern Ethiopia: A School Based Cross-Sectional Study. American Journal of Health Research.Vol. 2, No. 5, pp. 271-274. DOI: 10.11648/j.ajhr.20140205.18
Yibeltal Aschale, Ligabaw Worku, Ayenew Addisu, Mekuannint Alemu and Abebe Alemu. Comparison of direct wet mount, Kato-katz and formol ether sedimentation technique for the diagnosis of hookworm infection in Debre Elias Woreda, Northwest Ethiopia. Tropical Doctor. 2020. DOI: 10.1177/0049475520975936
Hoàng Thuý Hằng, Nguyễn Hữu Ngọc Tuấn, Nhữ Thị Hoa. 2019. Giá trị chẩn đoán của các kỹ thuật xét nghiệm phân trong nhiễm giun móc ở học sinh cấp 1, huyện Củ Chi, Thành phố Hồ Chí Minh, Y Học TP. Hồ Chí Minh. Phụ Bản Tập 23. Số 3. Tr. 400
Hamida Khanum, Mafruha Alam, Imtiaz Khalil and Rashidul Haque. 2015. Quantative assesment of kato‐katz thick smear and formol‐ether concentration technique for the Diagnosis Of Ascaris Lumbricoides. Dhaka Univ. J. Biol. Sci. 24(1): 65‐72, 2015 (January)
Mengistu Endris, Zinaye Tekeste, Wossenseged Lemma, and Afework Kassu. Comparison of the Kato-Katz, Wet Mount, and Formol-Ether Concentration Diagnostic Techniques for Intestinal Helminth Infections in Ethiopia. Hindawi Publishing Corporation. Volume 2013, Article ID 180439, 5 pages. http://dx.doi.org/10.5402/2013/180439 http://dx.doi.org/10.5402/2013/180439">
Tadesse Hailu and Bayeh Abera. Performance evaluation of direct saline stool microscopy, Formol ether concentration and Kato-Katz diagnostic me thods for intestinal parasitosis in the absence of gold standard methods. Tropical Doctor. 2015. DOI: 10.1177/0049475515581127
Abebe Fenta, Tadesse Hailu Jember, Megbaru Alemu Abate and Endalkachew Nibret. 2019. Evaluating the Performance of Diagnostic methods for soil transmitted helminths against the “Gold” standard in the Amhara National Regional State, Northwest Ethiopia. Research Square. DOI: 10.21203/rs.3.rs-30993/v2
Solomon Taye. 2014. Comparison of Kato-Katz and Formol-Ether Concentration Methods for the Diagnosis of Intestinal Helminthic Infections among School Children of Wonji Shoa Town, Eastern Ethiopia: A School Based Cross-Sectional Study. American Journal of Health Research.Vol. 2, No. 5, pp. 271-274. DOI: 10.11648/j.ajhr.20140205.18
Benjamin Speich, Stefanie Knopp, Khalfan A Mohammed, I Simba Khamis, Laura Rinaldi, Giuseppe Cringoli, David Rollinson and Jürg Utzinger. 2010. Comparative cost assessment of the Kato-Katz and FLOTAC techniques for soil-transmitted helminth diagnosis in epidemiological surveys. Speich et al. Parasites & Vectors. Article number: 71
Hugo C. Turner, Alison A. Bettis, Julia C. Dunn, Jane M. Whitton, T. Déirdre Hollingsworth, Fiona M. Fleming and Roy M. Anderson. Considerations for moving beyond the Kato-katz technique parasites as we move towards elimination. Trends in Parasitology, June 2017, Vol. 33, No. 6, DOI: http://dx.doi.org/10.1016/j.pt.2017.01.007 http://dx.doi.org/10.1016/j.pt.2017.01.007">